Project Roadmap
For a typical monoclonal antibody project (Abmart will provide a custom Project Roadmap for each individual project.)
Steps
Reports

1.Project Preparation

Customer to provide:

Plasmid DNA clone encoding full length target protein

 

 

 

2.Antigen Preparation

Protein expression and

purification

  Antigen report

3.Antibody Preparation

Animal

Immunization

Animals: 6 mice

Dose: 50 ug/animal                   Schedule (weeks): 3/2/2/2

Anti-sera ELISA Report

Monoclonal Antibody Screening

Fusion of the mouse spleen cells;

Hybridoma selection

 

Antibody Preparation

Ascites production;

Protein G affinity purification ;

Antibody lyopholization

 

4.Antibody Characterization

ELISA  detection

Antibody ELISA Report

Total:  5-7 months


For a typical polyclonal antibody project (Abmart will provide a custom Project Roadmap for each individual project.)
Steps
Reports
1.Project Preparation
Customer to provide:

Plasmid DNA clone encoding full length target protein
 
2.Antigen Preparation
Protein expression and
purification
   Antigen Report
3.Antibody Preparation
Animal
Immunization
2 Rabbits
Dose :200ug/rabbit
Schedule(weeks): 1/2/2

Anti-sera ELISA Report

Antibody Preparation  
Bleeding;
Protein A/G  purification
 
Antibody Freeze-dry
 
 
4.Antibody Characterization
ELISA  detection

Antibody ELISA Report

Total:  3-4 months